Device

Part:BBa_K2326004:Design

Designed by: Yantong Wang   Group: iGEM17_BNDS_China   (2017-10-23)


Overexpression of gadA with super ribosomal bonding side controlling with lactose inducible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1711
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1434
    Illegal AgeI site found at 1513
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3463


Design Notes

LacI-Ptac-B0034-gadA-B0034-E0040-terminator


Source

Escherichia coli str. K-12 substr. MG1655; Lactobacillus brevis

References